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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in lots of tumors, which results in chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was located to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important role in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be explained. Two more splice variants had been claimed but detailed nucleotide sequence information and facts will not be but offered.
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Blocking peptides are peptides that bind especially to the goal antibody and block antibody binding. These peptide usually includes the epitope regarded with the antibody.
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This guidebook outlines further more attainable optimization of cellular and immunoassay parameters to make sure the very best results are...
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates in the G1-S phase boundary and is particularly degraded as cells progress by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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